Rock River Laboratory Mold IdentificationAnti-Nutritional Measures

Manage the risks posed by bacteria, mold, yeast, and toxins through count, identification, and screening options

Bacteria, mold, yeast, and toxins are in a class all of their own, and by wrecking havoc on animal feeds, they've earned the category name of "anti-nutritional factors". Managing the risks associated with these organisms is possible through counts, identification, and screening as soon as there is suspect. Rock River Laboratory offers basic and advanced analysis for bacteria, mold, yeast, and toxins, while also partnering with other laboratories for more involved needs. Analyses offered, along with guidelines for resulting count levels can be reviewed below. Learn more about the beginnings and effects of these anti-nutritional factors in this article from Progressive Dairyman.


Bacteria, Mold, Yeast, and Toxin Analyses

  • TMR Hygiene Diagnostic package
  • Yeast and mold count: Requires a 5-day incubation
  • Yeast and mold count and identification
  • Rapid mold and yeast count: For those needing counts quickly, this analysis provides mold and yeast levels after a 2-day incubation, but does not offer identification.
  • Clostridium perfringens count: Also identifies the combination of Enterococcus faecalis and Salmonella typhimurium, which are undesirable enterobacteria
  • Toxin analysis: DON, Zearalenone, T-2, Fumonisin, Aflatoxin, or Ochratoxin-A
  • Fusarium mycotoxin screen: Scans for 13 toxins from Fusarium
  • Basic mycotoxin screen: Scans for 12 toxins from Fusarium and Aflatoxin
  • Comprehensive mycotoxin Screen: Scans for 19 toxins from Fusarium and Citrinin along with basic toxins

Guidelines for Resulting Levels of Mold, Yeast, and Toxins

Mold, Yeast, and Clostridium perfringens Guidelines for Agricultural Feed and Total Mixed Rations

Mycotoxin Guidelines and Dietary Limits


The Details on Mycotoxin Analyses

When it comes to analyzing feed and feed products for anti-nutritional substances beyond yeast and mold, Rock River Laboratory offers a variety of mycotoxin analysis packages. There is a boundless amount of information regarding which toxins, at what levels, cause certain ailments.  The focus here is to help you decide which package is right for you based on your identifying needs.

The RIDASCREEN® toxin analysis is an antibody kit designed for a quick determination of single toxins. These tests are done on Mondays and Thursdays by our own technicians at the Central Laboratory in Wisconsin. If you are seeing any toxin-producing molds in your feeds, or feed refusals, you can pick and choose which toxins you would like to analyze for. Keep in mind turnaround time is based on what day of the week you are submitting your sample. Call our team at 920-261-0446 for detailed pricing on each toxin. 

RIDASCREEN® Analysis

Choose individual toxin analysis

Analyte

Limit of Detection
(based on standard)

Deoxynivalenol (DON)

<0.2 ppm

Zearalenone

17-41 ppb

T-2

<20 ppb

Fumonisin

0.22 ppm

Aflatoxin

<1.7 ppb

Ochratoxin-A

5 ppb

For a larger spectrum of toxins, Rock River Laboratory offers four different screen packages. These screens are designed for a snapshot of the toxins that may be present within the feed.

The Basic Mycotoxin Screen is just as is states, and includes common toxins produced by Fusarium and Aspergillus molds. The Fusarium Mycotoxin Screen reports 13 different toxins that are specific to being produced by Fusarium molds. The Comprehensive Mycotoxin Screen is the largest list of reported toxins at 19 - a good fit if you are looking to identify any and all toxins that may be present within a feed.

Our newly added Common Mycotoxin Screen lists 7 of our commonly-asked-for toxins, at varied detection limits, for an affordable cost. Also newly available is the Aflatoxin Mycotoxin Screen, which breaks down aflatoxin presence into four further identifications. For pricing on each type of screen, call our team at 920-261-0446. 

Comprehensive Mycotoxin Screen

19 toxins included

Analyte

Limit of Detection

Method

15-Acetyl Deoxynivalenol

0.1 ppm

LCMSMS

3-Acetyl Deoxynivalenol

0.1 ppm

LCMSMS

Aflatoxin B1

1 ppb

LCMSMS

Aflatoxin B2

1 ppb

LCMSMS

Aflatoxin G1

1 ppb

LCMSMS

Aflatoxin G2

1 ppb

LCMSMS

Citrinin

50 ppb

LCMSMS

Diacetoxyscirpenol

20ppb

LCMSMS

Deoxynivalenol

0.1 ppm

HPLC

Fumonisin B1

0.1 ppm

LCMSMS

Fumonisin B2

0.1 ppm

LCMSMS

Fumonisin B3

0.1 ppm

LCMSMS

Fusarenon X

0.1 ppm

LCMSMS

HT-2 Toxin

5 ppb

LCMSMS

Neosolaniol

20 ppb

LCMSMS

Nivalenol

0.1 ppm

LCMSMS

Ochratoxin A

1 ppb

LCMSMS

T-2 Toxin

5 ppb

LCMSMS

Zearalenone

50 ppb

HPLC

 

Common Mycotoxin Screen

7 toxins included

Analyte

Limit of Detection

Method

Aflatoxin B1

2.5 ppb

LCMSMS

Aflatoxin B2

2.5 ppb

LCMSMS

Aflatoxin G1

2.5 ppb

LCMSMS

Aflatoxin G2

2.5 ppb

LCMSMS

Deoxynivalenol

250 ppb

LCMSMS

T-2 Toxin

100 ppb

LCMSMS

Zearalenone

250 ppb

LCMSMS

Basic Mycotoxin Screen

11 toxins included

Analyte

Limit of Detection

Method

Aflatoxin B1

1 ppb

LCMSMS

Aflatoxin B2

1 ppb

LCMSMS

Aflatoxin G1

1 ppb

LCMSMS

Aflatoxin G2

1 ppb

LCMSMS

Deoxynivalenol

0.1 ppm

LCMSMS

Fumonisin B1

0.1 ppm

LCMSMS

Fumonisin B2

0.1 ppm

LCMSMS

Fumonisin B3

0.1 ppm

LCMSMS

Ochratoxin A

1 ppb

LCMSMS

T-2 Toxin

5 ppb

LCMSMS

Zearalenone

12.5 ppb

LCMSMS

Fusarium Mycotoxin Screen

13 toxins produced by Fusarium

Analyte

Limit of Detection

Method

15-Acetyl Deoxynivalenol

0.1 ppb

GC

3-Acetyl Deoxynivalenol

0.1 ppb

GC

Deoxynivalenol

0.1 ppb

GC

Diacetoxyscirpenol

100 ppb

GC

Fumonisin B1

0.1 ppm

HPLC

Fumonisin B2

0.1 ppm

HPLC

Fumonisin B3

0.1 ppm

HPLC

Fusarenon X

0.1 ppm

GC

HT-2 Toxin

100 ppb

GC

Neosolaniol

100 ppb

GC

Nivalenol

0.1 ppm

GC

T-2 Toxin

100 ppb

GC

Zearalenone

50 ppb

HPLC

 

Aflatoxin Mycotoxin Screen

4 Aflatoxins identified

Analyte

Limit of Detection

Method

Aflatoxin B1

1 ppb

HPLC

Aflatoxin B2

1 ppb

HPLC

Aflatoxin G1

1 ppb

HPLC

Aflatoxin G2

1 ppb

HPLC

 

 

Rock River Laboratory

Founded in 1976, Rock River Laboratory is a family-owned laboratory network that provides production assistance to the agricultural industry through the use of advanced diagnostic systems, progressive techniques, and research-supported analyses.  Employing a team of top specialists in their respective fields, Rock River Laboratory provides accurate, cost-effective, and timely analytical results to customers worldwide, while featuring unsurpassed customer service.

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